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Product Details
Source
PE-Labeled Monoclonal Mouse IgG1 Antibody Isotype Control is produced via site-specific conjugation of PE to Monoclonal Mouse IgG1 Antibody Isotype Control under optimal conditions with a proprietary technology.
Application
Flow Cytometry. Please note that this product is NOT compatible to streptavidin detection system.
Species
Mouse
Isotype
Mouse IgG1/kappa
Specificity
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins, which is normally not present in Humans or animals. Therefore, DNP-PM1 does not react with any antigen of Human or animal cells.
Immunogen
DNP (Dinitrophenyl)
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Recommended Dilution
1:50
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.5% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
Bioactivity-FACS
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Mouse IgG1 Antibody Isotype Control (Cat. No. DNP-PM1) and positive control PE-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).
Protocol
5e5 of PBMCs were stained with a series of concentrations of PE-Labeled Monoclonal Mouse IgG1 Antibody Isotype Control (Cat. No. DNP-PM1), and PE signal was used to evaluate the binding activity (QC tested).
Protocol
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[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
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