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Product Details
Source
Anti-Human IgG Antibody-Acridinium ester is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
Species
Mouse
Isotype
Mouse IgG
Conjugate
Acridine ester
Antibody Type
Hybridoma Monoclonal
Specificity
This product is a specific antibody specifically reacts with IgG.
Purity
95% as determined by SDS-PAGE.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH6.3.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Anti-Human IgG Antibody-Acridinium ester on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at increasing concentration coupled to Anti-Human IgG Antibody-Acridinium ester (Cat. No. AHG-Y69, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-Human IgG Antibody-Acridinium ester (Cat. No. AHG-Y69) is stable at 37°C for 48 hours.
Protocol
The MPCLIA assay shows that Anti-Human IgG Antibody-Acridinium ester (Cat. No. AHG-Y69) is stable after freezing and thawing 3 times.
Protocol
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Background
The antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, cynomolgus and bovine serum proteins, but it is not sure if the product cross-react with immunoglobulins from other species.
Acridinium ester is one of the most commonly used excited substrates for protein labeling and widely used in automated instruments because of its high sensitivity with detection limits in the attomole range. Acridinium ester can be triggering by chemiluminescent substrate solution and generate intense signal, the result signals (relative light units, RLU) can be measured at 430 nm.
The Anti-human IgG-Acridine Ester is easy to capture the human IgG, and the bounded human IgG have no activity lost, this ready to use products could greatly save your protein labeling time and hassle, and help us get the best performance and highly reproducible results.
Recent Advances
Drug Development Progress- English Name：
Immunoglobulin G
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Approved
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