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Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell

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SCJUR-STF178-2Vials (1Vial X 2)

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Product Details
Performance Data
Reviews & Citations
Important Statement
Resources

Advantages

Genetically modified cell lines best reflect MOA (Mechanism of Action)
Higher activity and larger assay window for robust and reproducible cell-based bioassay
Comprehensive application data to support assay development and validation
Full tracible record, stringent quality control and validated cell passage stability
Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
Global commercial license assistance whenever regulatory filing is required

Description

The Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was engineered to not only express the NF-κB signaling response element, but also express the receptor human DR3 (TL1A receptor) (Uniprot: Q93038-1). When stimulated with human TL1A protein, the TL1A/DR3 interaction drives NF-κB-mediated luminescence. Inhibition of TL1A binding to DR3 by either anti-TL1A or anti-DR3 antibodies results in a decrease in luminescence.

Application

• Screen for neutralizing antibodies blocking the stimulation of human TL1A protein.

DR3 Assay Principles

Growth Properties

Suspension

Selection Marker

Hygromycin B (20 μg/mL)

Complete Growth Medium

RPMI-1640 + 10% FBS

Freeze Medium

Serum-free cell cryopreservation medium

Quantity

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Storage

Frozen in liquid nitrogen.

Mycoplasma Testing

Negative

Sterility Testing

Negative

Instructions for Use

See data sheet for detailed culturing and assay protocol.

ACRO Quality Management System

QMS(ISO, GMP)

Quality Advantages

Quality Control Process

Application

Inhibition of human TL1A protein-induced reporter activity.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human TL1A protein (Cat. No. TLA-H5243) with a final concentration of 0.02 μg/mL. The EC50 of anti-human TL1A neutralizing antibody is approximately 0.018 μg/mL.

Protocol

Signaling Bioassay

Response to human TL1A protein (RLU).
The Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was incubated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). The parental reporter cell NF-kB (Luc) Jurkat Reporter Cell (Cat. No. SCJUR-STF113) lacking human DR3 expression was used as the control. The EC50 of human TL1A protein determined on Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was approximately 0.014 μg/mL. Compared to the Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell, the parental reporter cell NF-kB (Luc) Jurkat Reporter Cell lacking human DR3 expression exhibited no activation response to human TL1A protein stimulation.

Protocol

Response to human TL1A protein (FOLD).
The Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was incubated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). The parental reporter cell NF-kB (Luc) Jurkat Reporter Cell (Cat. No. SCJUR-STF113) lacking human DR3 expression was used as the control. The max induction fold of human TL1A protein determined on Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was approximately 54.12. Compared to the Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell, the parental reporter cell NF-kB (Luc) Jurkat Reporter Cell lacking human DR3 expression exhibited no activation response to human TL1A protein stimulation.

Protocol

Passage Stability

Passage stability analysis by Signaling Bioassay.
The continuously growing Human DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TL1A protein (Cat. No. TLA-H5243). Human TL1A protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 10-23.

Protocol

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