Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell

Expression analysis of human GFRAL on Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell or negative control cell using anti-human GFRAL antibody followed by staining with APC anti-human IgG Fc antibody.

Inhibition of human GDF15 protein-induced reporter activity by anti-human GDF15 neutralizing antibody.
The Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell was incubated with serial dilutions of antibodies in the presence of GDF15 protein (Cat. No. GD5-H5149) with a final concentration of 0.3 μg/mL. The EC50 of anti-human GDF15 neutralizing antibody (Ponsegromab) is approximately 0.5928 μg/mL.

Inhibition of human GDF15 protein-induced reporter activity by anti-human GFRAL neutralizing antibody.
The Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell was incubated with serial dilutions of antibodies in the presence of GDF15 protein (Cat. No. GD5-H5149) with a final concentration of 0.3 μg/mL. The EC50 of anti-human GFRAL neutralizing antibody (NGM-120) is approximately 1.285 μg/mL.

Response to human GDF15 protein (RLU).
The Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human GDF15 protein (Cat. No. GD5-H5149). The EC50 was approximately 0.03276 μg/mL.

Response to human GDF15 protein (FOLD).
The Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human GDF15 protein (Cat. No. GD5-H5149). The max induction fold was approximately 54.06.