Human IL-10 R alpha/IL-10 R beta (Luc) HEK293 Reporter Cell

Co-expression analysis of human IL-10 R alpha and IL-10 R beta on Human IL-10 R alpha/IL-10 R beta (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human IL-10 R alpha/IL-10 R beta (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti- IL-10 R alpha antibody and APC-labeled anti- IL-10 R beta antibody.

Inhibition of human IL-10 protein-induced reporter activity by anti-human IL-10 neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human IL-10 protein (Cat. No. IL0-H5219) with a final concentration of 0.004 μg/mL. The EC50 of anti-human IL-10 neutralizing antibody is approximately 0.4839 μg/mL.

Response to human IL-10 protein (RLU).
The Human IL-10 R alpha/IL-10 R beta (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-10 protein (Cat. No. IL0-H5219). The EC50 was approximately 0.0135 μg/mL.

Response to human IL-10 protein (FOLD).
The Human IL-10 R alpha/IL-10 R beta (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-10 protein (Cat. No. IL0-H5219). The max induction fold was approximately 240.

Passage stability analysis by Signaling Bioassay.
The continuously growing Human IL-10 R alpha/IL-10 R beta (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-10 protein. Human IL-10 protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 5-20.