NF-κB (Luc) HEK293 Reporter Cell

Inhibition of human TNFα protein-induced reporter activity by anti-human TNFR1 neutralizing antibody.
The NF-kB (Luc) HEK293 Reporter Cell was incubated with serial dilutions of antibodies in the presence of human TNFα protein (Cat. No. TNA-H4211) with a final concentration of 0.0006 μg/mL. The EC50 of anti-human TNFR1 neutralizing antibody (GSK1995057) is approximately 0.00174 μg/mL.

Inhibition of human TNFα protein-induced reporter activity by anti-human TNFα neutralizing antibody.
The NF-kB (Luc) HEK293 Reporter Cell was incubated with serial dilutions of antibodies in the presence of human TNFα protein (Cat. No. TNA-H4211) with a final concentration of 0.0006 μg/mL. The EC50 of anti-human TNFα neutralizing antibody (Adalimumab) is approximately 0.01117 μg/mL.

Response to human TNFα protein (RLU).
The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TNFα protein (Cat. No. TNA-H4211). The EC50 was approximately 0.64 ng/mL.

Response to human TNFα protein (FOLD).
The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TNFα protein (Cat. No. TNA-H4211). The max induction fold was approximately 235.

Passage stability analysis by Signaling Bioassay.
The continuously growing NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TNFα protein (Cat. No. TNA-H4211). Human TNFα protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 8-26.