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CelThera™ GMP T Cell Expansion Medium (Phenol Red-free)

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GMP-CM3102-1L

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Components

Cat. No.	Components	Size
GMP-CM3102A	CelThera™ GMP Immune Cell Expansion Medium (Phenol Red-free)	1000 mL
GMP-CM3101-1	CelThera™ GMP T Cell Expansion Supplement	7.25 mL

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Product Details

CelThera™ GMP T Cell Expansion Medium (Phenol Red-free) is a serum-free culture medium specifically developed to support human T cell culture. It is a serum-free, animal origin-free T cell maintenance and expansion medium produced under GMP conditions.

Compared to traditional culture media or xeno-free culture media, animal origin-free culture media can better reduce the risk of introducing potential pathogenic microorganisms during culture process, improve batch-to-batch consistency, and prevent T cell overactivation by undefined components in the serum.

CelThera™ GMP T Cell Expansion Medium (Phenol Red-free) does not require the addition of any serum or serum replacements and also maintains the high fold expansion of T cells. If users choose to add serum or serum replacement, the dosage should be determined by specific T cell applications.

Features

Serum-free, animal origin-free (AOF), and exogenous growth factors free.
Designed to support low-density seeding and high fold expansion of T cells.
Suitable for large-scale T cell expansion.
No additional serum or serum replacements needed.
T cell phenotypes similar to media supplemented with serum or serum replacement.
Contains only recombinant proteins as components, no antibiotics added during manufacturing.
Produced according to current GMP guidelines.

Storage

The CelThera™ GMP Immune Cell Expansion Medium (Phenol Red-free) is stable for 18 months when stored under 2-8°C, protect from light.
The CelThera™ GMP T Cell Expansion Supplement is stable for 24 months when stored under -20°C or below, protect from light.

Shipping

Components may ship in separate packages with different temperature controls (dry ice or blue ice). Please inquire about the shipping cost. Inspect all parcels upon arrival.

ACRO Quality Management System

QMS(ISO, GMP)

Quality Advantages

Quality Control Process

Application Data

T cell expansion rate and cell viability in various media.
T cells from PBMCs of 3 different donors were activated and cultured for 11 days in various media supplemented with 300 IU/ml Acro GMP IL-2 (Cat. No. GMP-L02H14). Cell count and viability were performed on day 6, day 8 and day 11 by trypan blue staining. It indicated that cells in Acro T cell medium (Cat. No. GMP-CM3102) had a faster proliferation rate and higher viability than that of the other two media.

Tcm ratio in various media.
T cells from PBMCs of 3 different donors were activated and cultured in various media supplemented with 300 IU/ml Acro GMP IL-2 (Cat. No. GMP-L02H14). Tcm percentage (CD45RO+/CCR7+) was determined by flow cytometry when cells reached about 50-fold expansion. It indicated that cells in Acro T cell medium (Cat. No. GMP-CM3102) had a higher percentage of central memory T cells than that of the other two media.

Lentivirus transduction efficiency in various media.
T cells from PBMCs were activated and cultured in various media supplemented with 300 IU/ml Acro GMP IL-2 (Cat. No. GMP-L02H14). 24 hours after activation, the cells were transduced with pLenti-CMV-EGFP-puro lentivirus (MOI=3 or 6). 24hrs after transduction, the lentivirus was removed by centrifugation. Then, the cells were cultured for 48hrs and the CD3+eGFP+ population was detected by flow cytometry. It indicated that cells in Acro T cell medium (Cat. No. GMP-CM3102) had a similar lentiviral transduction efficiency to that of the other medium.

Multiple Donor Verification

The CelThera™ GMP T Cell Expansion Medium (Phenol Red-free) effectively promotes the expansion of T cells in PBMCs.
Human PBMCs were cultured with GMP Human IL-2 Protein (ACRO, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACRO, Cat. No. GMP-CM3102) or T cell culture medium (Company L +2.5% SR) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium (ACRO) can be comparable with the Company L +2.5% SR. Notably, the cells exhibits better expansion in CelThera™ GMP T Cell Expansion Medium (ACRO, Cat. No. GMP-CM3102).

Large-scale Culture Verification

The CelThera™ GMP T Cell Expansion Medium (Phenol Red-free) effectively promotes the expansion of T cells in PBMCs.
Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACRO, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACRO, Cat. No. GMP-MC2824), cultured with CelThera™ GMP T Cell Expansion Medium (ACRO, Cat. No. GMP-CM3102) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACRO, Cat. No. GMP-L02H14) for two weeks.
The results showed that GMP Human IL-2 Protein, GMP Monoclonal Anti-Human CD3 Antibody (OKT3), GMP Monoclonal Anti-Human CD28 Antibody and CelThrea™ GMP T Cell Expansion Medium could be used to culture T cells in a 3L large system. It can efficiently expand cells with high viability. (n=3 technical replicates)