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CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free)

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Transfer Cap for Square Media Bottle (PCA-25021401) is available for convenient sterile transfer of media from the bottle to closed culture systems like cell culture bags via aseptic welding.

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GMP-GDTCM32-1L

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Components

Cat. No.	Components	Size
GMP-CM3102A	CelThera™ GMP Immune Cell Expansion Medium (Phenol Red-free)	1000 mL
GMP-CM3101-1	CelThera™ GMP T Cell Expansion Supplement	7.25 mL
GMP-CM31S3	CelThera™ GMP Immune Cell Supplement D	1.5 mL

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Product Details

CelThera™ GMP GDT Cell Expansion Medium (Phenol Red-free) is a serum-free culture medium specifically developed to support human gamma delta T cell culture. It is a serum-free, animal origin-free T cell maintenance and expansion medium produced under GMP conditions.

Compared to traditional culture media or xeno-free culture media, animal origin-free culture media can better reduce the risk of introducing potential pathogenic microorganisms during culture process, and improve batch-to-batch consistency.

Features

Chemically Defined, animal origin-free (AOF), and exogenous growth factors free;
Provide unique nutritional requirements that could supply a suitable growth environment for gamma delta T cells;
Designed to support low-density seeding and high-density seeding of gamma delta T cell expansion;
A better performance compared to other commercial products;
Contains only recombinant proteins as components, no antibiotics added during manufacturing;
Produced according to current GMP guidelines.

Storage

CelThera™ GMP Immune Cell Expansion Medium (Phenol Red-free) is stable for 18 months when stored under 2-8°C, protect from light.
CelThera™ GMP T Cell Expansion Supplement is stable for 24 months when stored under -20°C or below, protect from light.
CelThera™ GMP Immune Cell Supplement D is stable for 30 months when stored under -20°C or below, protect from light.

Shipping

Components may ship in separate packages with different temperature controls (dry ice or blue ice). Please inquire about the shipping cost. Inspect all parcels upon arrival.

ACRO Quality Management System

QMS(ISO, GMP)

Quality Advantages

Quality Control Process

Application Data

The CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free/Phenol Red) enables high-efficiency expansion of GDT cells from PBMCs.
PBMCs were cultured for two weeks with the CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free/Phenol Red) (ACROBiosystems, Cat. No. GMP-GDTCM32/GMP-GDTCM33). The results of the cell expansion showed that GDT Cell Expansion Medium (ACROBiosystems) can be comparable to the commercially available products. Notably, the total cells and γδ2T cells exhibit better expansion in ACRO’s GDT Cell Expansion Medium. Data represent means ± SD (n=3).

The CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free/Phenol Red) enables high-efficiency expansion of GDT cells from PBMCs.
PBMCs were cultured for two weeks with the CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free/Phenol Red) (ACROBiosystems, Cat. No. GMP-GDTCM32/GMP-GDTCM33). The cell viability results showed that GDT Cell Expansion Medium (ACROBiosystems) can be comparable to commercially available products. The results of γδ2T proportion showed no significant difference. Notably, the cells exhibit better cell viability in ACRO’s GDT Cell Expansion Medium. Data represent means ± SD (n=3).

The CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free/Phenol Red) enables high-efficiency expansion of GDT cells from PBMCs.
PBMCs were cultured for two weeks with the CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free/Phenol Red) (ACROBiosystems, Cat. No. GMP-GDTCM32/GMP-GDTCM33) supplemented with human AB serum. The results of the Cell expansion showed that GDT Cell Expansion Medium (ACROBio) can be comparable to the commercially available products. Notably, the total cells and γδ2T cells exhibit better expansion in ACRO’s GDT Cell Expansion Medium. Data represent means ± SD (n=3).

The CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free/Phenol Red) enables high-efficiency expansion of GDT cells from PBMCs.PBMCs were cultured for two weeks with the CelThera™ GMP GDT Cell Expansion Kit (Phenol Red-free/Phenol) (ACROBiosystems, Cat. No. GMP-GDTCM32/GMP-GDTCM33) supplemented with human AB serum. The cell viability results showed that GDT Cell Expansion Medium (ACROBiosystems) can be comparable to the commercially available products. Notably, the cells exhibit better cell viability in ACRO’s GDT Cell Expansion Medium compared to commercially available products. Data represent means ± SD (n=3).

Large-scale Culture Verification

Starting with 9 million cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) as seed cells, continuous feeding and expansion were performed beginning from a 3 ml volume. After 14 days, the culture expanded to 2*1000 ml, yielding over 6 billion cells.