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Mouse Complement C5a Protein, His Tag

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Cat. No. / Size
Price
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C5A-M51H4-100ug
$360.00
C5A-M51H4-1mg (250ug X 4)
$2615.00
ETA of in-stock products:2 business days
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Product Details

  • Synonym

    C5a, Complement Component 5a

  • Source

    Mouse Complement C5a, His Tag (C5A-M51H4) is expressed from E. coli cells. It contains AA Asn 679 - Arg 755 (Accession # P06684).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Complement C5a Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 10.9 kDa. The protein migrates as 14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Complement C5a SDS-PAGE

    Mouse Complement C5a, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Complement C5a ELISA

    Immobilized Mouse Complement C5a, His Tag (Cat. No. C5A-M51H4) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).

    Protocol

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Background

Complement component C5a (C5a) is a protein fragment generated upon cleavage of complement component C5. It is a potent chemotactic factor for human peripheral blood neutrophils and monocytes and is believed to play an important role in a number of inflammatory conditions. The major functions of C5a are as follows: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is an effective leukocyte chemoattractant, promoting the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing integrin avidity and upregulating the lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator and appears to be a key factor in the pathogenesis of many inflammatory diseases involving the complement system; and C5a modulates the balance between activating and inhibitory IgG Fc receptors on leukocytes, a mechanism that may enhance autoimmune responses under pathological conditions.

Recent Advances

 
Drug Development Progress
  • English Name:

    Complement C5a

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    2 Details

  • Highest Development Stage:

    Approved

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