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Human IL-17 RE (155-454) Protein, Fc Tag

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Cat. No. / Size
Price
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ILE-H5256-100ug
$360.00
ILE-H5256-1mg (200ug X 5)
$2615.00
ETA of in-stock products:2 business days
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Product Details

  • Synonym

    IL-17 RE, IL17RE, IL-17RE, IL-17 receptor E

  • Source

    Human IL-17 RE (155-454), Fc Tag (ILE-H5256) is expressed from human 293 cells (HEK293). It contains AA Thr 155 - His 454 (Accession # Q8NFR9-1).

    Predicted N-terminus: Thr 155

    Request for sequence
  • Molecular Characterization

    IL-17 RE Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 60.5 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-17 RE SDS-PAGE

    Human IL-17 RE (155-454), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     IL-17 RE ELISA

    Immobilized Human IL-17C, His Tag (Cat. No. ILC-H52H7) at 2 μg/mL (100 μL/well) can bind Human IL-17 RE (155-454), Fc Tag (Cat. No. ILE-H5256) with a linear range of 1-10 ng/mL (QC tested).

    Protocol
  • Bioactivity-BLI

     IL-17 RE BLI

    Loaded Human IL-17 RE (155-454), Fc Tag (Cat. No. ILE-H5256) on Protein A Biosensor, can bind Human IL-17C, His Tag (Cat. No. ILC-H52H7) with an affinity constant of 0.714 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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Background

Interleukin-17 receptor E (IL-17 RE) is an orphan receptor of the IL-17 receptor family, also known as UNQ3056, PRO9877, IL-17 Receptor E. IL-17RE is known to be expressed in the pancreas, brain, and prostate. IL-17RE is a receptor specific to IL-17C and has an essential role in host mucosal defense against infection. IL-17C functioned in a unique autocrine manner, binding to a receptor complex consisting of the receptors IL-17RA and IL-17RE, which was preferentially expressed on tissue epithelial cells. Some data identify IL-17RE as a receptor of IL-17C that regulates early innate immunity to intestinal pathogens.

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