Nipah virus Pre-Fusion glycoprotein, His Tag (MALS verified)

Synonym

Prefusion glycoprotein F0/pre-F protein (NiV)

Source

Nipah virus Pre-Fusion glycoprotein, His Tag (FUN-N52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Ser 487 (Accession # Q9IH63-1).

Predicted N-terminus: Ile 27

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 55.9 kDa. The protein migrates as 57-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a trimer.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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