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Product Details
Synonym
STEAP1, PRSS24, STEAP
Source
Human STEAP1 Full Length Protein, Twin-Strep,His Tag (ST1-H5283) is expressed from human 293 cells (HEK293). It contains AA Glu 2 - Leu 339 (Accession # Q9UHE8-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a twin strep tag at the N-terminus and a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 44.7 kDa. The protein migrates as 40-42 kDa and 80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>85% as determined by SDS-PAGE.
Formulation
This product is not suitable for cell based experiments due to cytotoxicity of detergent.
Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove detergent.
Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Buffer A, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
- *The Buffer A (Cat. No. DN-12) is sold separately and not included in protein. You can learn about the product information through the link. We recommend DN-12 for ELISA assay and DC-11 for SPR/BLI assay.
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Performance Data
SDS-PAGE
Human STEAP1 Full Length Protein, Twin-Strep,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human STEAP1 Full Length Protein, Twin-Strep,His Tag (Cat. No. ST1-H5283) at 2 μg/mL (100 μL/well) can bind Vandortuzumab biosimilar with a linear range of 4-63 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-STEAP (Vandortuzumab) captured on Protein A Chip can bind Human STEAP1 Full Length Protein, Twin-Strep,His Tag (Cat. No. ST1-H5283) with an affinity constant of 2.30 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (QC tested).
Protocol
Bioactivity-BLI
Loaded Anti-STEAP1(Xaluritamig) on Protein A Biosensor, can bind Human STEAP1 Full Length Protein, Twin-Strep,His Tag (Cat. No. ST1-H5283) with an affinity constant of 9.86 nM as determined in BLI assay (in presence of DDM and CHS) (ForteBio Octet RED96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Six-transmembrane epithelial antigen of the prostate 1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Phase 3 Clinical
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