English

1/6

+3 View all

COA

MSDS

Streptavidin Protein Europium chelate (for TR-FRET)

For detailed protocols, please download the TR-FRET Toolbox Experimental Guide.

1/6

+3 View all

COA

MSDS

Cat. No. / Size

Price

Availability

Qty

FRT-TD09-1000tests

$152.00

Inquire about delivery time

FRT-TD09-5000tests

$354.00

In Stock - Delivery in 2-3 business days

FRT-TD09-20000tests

$1,132.00

In Stock - Delivery in 2 weeks

Try our Toolbox today and build your own TR-FRET system. Claim your trial now!

Sub-Total:

$ 0

Add to Cart

Request Quote

Product Details
Performance Data
Reviews & Citations
Important Statement
Resources

Application

The Streptavidin Protein Europium chelate is a universal tool for TR-FRET assays that can bind biotinylated molecules. This product uses high-purity streptavidin (SA) covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.

Product Specifications

Source	This Streptavidin Protein is expressed from E. coli cells. It contains AA Ala 37 - Ser 163 (Accession # P22629-1).
Formulation	Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Physical Appearance	Powder
Storage (unopened)	-20°C to -70°C
Storage (opened)	-70°C
Unit Size	5000 tests & 20000 tests
Technology	TR-FRET
Intended Use	For binding the biotinylated molecules as donor in TR-FRET Assay

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

ACRO Quality Management System

QMS(ISO, GMP)

Quality Advantages

Quality Control Process

Activity-TR-FRET

Please refer to DS document for the assay protocol.

In the TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4), at 1 ng/well, can bind to the Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear detection range of 5-0.005 μg/mL (QC tested).

In the TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (Cat. No. FRT-TA04) is used as the Acceptor. An inhibition Assay was performed to evaluate the interaction between Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) and Human PD-L1, His Tag (Cat. No. PD1-H5229) using Monoclonal Anti-Human PD-1 Antibody, Human IgG4 resulting in a typical IC50 of 2.285 μg/mL (Routinely tested).

In the TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-His Tag Antibody, Mouse IgG1 FA-Labeled (Cat. No. FRT-TA05) is used as the Acceptor. An inhibition Assay was performed to evaluate the interaction between Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) and Human PD-L1, His Tag (Cat. No. PD1-H5229) using Monoclonal Anti-Human PD-1 Antibody, Human IgG4 resulting in a typical IC50 of 1.578 μg/mL (Routinely tested).

In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3), at 1.25 ng/well, can bind to the Cetuximab with a linear detection range of 8-0.001 μg/mL (Routinely tested).

In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2), at 1.25 ng/well, can bind to the Pertuzumab with a linear detection range of 4-0.001 μg/mL (Routinely tested).

In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human CD20 Full Length, His,Avitag (Cat. No. CD0-H82E3), at 1.5 ng/well, can bind to the Glofitamab with a linear detection range of 16-0.008 μg/mL (Routinely tested).