Fluorescent DLL3 supports direct flow-cytometric detection of DLL3-binding antibodies or engineered cells. DLL3-coupled beads are useful for binder capture, enrichment, or multivalent antigen presentation, while unconjugated protein remains preferable for quantitative kinetics.

Use recombinant DLL3 for controlled affinity and epitope screening, then confirm hits on a DLL3 stable cell line. This filters candidates that recognize an isolated or tag-dependent epitope but poorly bind cell-surface DLL3.