I used this antibody as a control for flow cytometry and it was very reliable. A clear peak shift could be seen with this antibody. When we had questions about what SARS-CoV-2 variants this mAb bound to, ACRO replied quickly and gave us information on the variants we inquired about and then some. ACRO is a company I would purchase from again and my company has used them consistently.
I used this antibody as a control for flow cytometry and it was very reliable. When we had questions about what SARS-CoV-2 variants this mAb bound to ACRO replied quickly and gave us information on the variants we inquired about and then some. ACRO is a company I would purchase from again.
We had purchased this antibody to be used in our in vitro pseudovirus neutralization assay. We had used it as a positive control for our assay. the assay worked very well and the antibody showed a dose dependent inhibition of the pseudovirus
ACRO always provides high-quality products in the face of the changing market, which greatly saves time for our R&D staff. The product currently has good stability for our testing and we hope that ACRO can develop more products for scientific applications going forward!
We have used this products in running out ELISAs to estimate the antibody levels in patient samples and the consistency in results was great. Easy to store the product. Most stable antigens in the market which can withstand slight temperature fluctuations. Long term stability. Was able to generate great results which are published already. Along this pandemic timeline, Acro is the only company which has been persistent with manufacturing all new mutants as soon as it is updated on CDC which helped a lot in understanding the mutant faster and generating data. Used widely in Inhibition ELISAs.
We placed an order for Omicron RBD, to run side-by-side analyses of our neutralizing antibodies against Omicron vs Wild type/Wuhan RBD protein (also from Acro) in biolayer interferometry experiments. AcroBio's Biotinylated Omicron RBD protein loads onto streptavidin biosensors very well - achieving a 1nm spectral shift within 40 seconds using a 100nM solution. We were able to obtain satisfactory results that were consistent with findings from other orthogonal methods for characterizing our antibodies (ELISA, viral infectivity, etc). Protein arrived in a timely manner.
This omicron protein is a really great product and is high quality. The protein came on time to conduct our experiments and we were able to produce satisfactory results from using this protein. The protein was shipped with dry ice and was in a secure package. We would highly recommend this company and this protein to other labs that are doing omicron research.
As a second year grad student at UW-Madison, I always choose ACRO as my first choice for proteins and other reagents. Every protein that we have ordered has been pure and yielded amazing results. I would highly recommend ACRO to anyone who wants to do solid fundamentally sound science.
Have ordered multiple spike protein RBD variants from ACRO, this product like all of them was superb. The ordering process was streamlined and efficient. Communication about shipping times and inquiries about any custom formulations were fantastic. The ability for the avi tag with site specific biotinylation is a game changer and acro does it fantastically!! Cannot recommend this product or any product from ACRO enough. Our favorite protein provider!
Ordered S1N-M122 as a positive control for ACE2 competition experiments with a panel of SARS-CoV-2 variant RBDs. This antibody worked incredibly well, binding the tested variant RBDs with high affinity. Additionally, this antibody blocked 100% of ACE2 binding to the RBDs and when paired with S1N-M130 they serve as important competition controls to ensure that the RBDs and ACE2 are behaving as expected. Overall would highly recommend this product.
Ordered AM130 as a binding control for ACE2 competition experiments with a panel of SARS-CoV-2 variant RBDs. This antibody worked incredibly well, showing strong binding (BLI) against all variants tested. As mentioned in the description, this is not a neutralizing antibody and it does not block ACE2, which made it a perfect binding control to ensure that the RBDs were in good shape and functional. Overall would highly recommend this product.
I ordered the Omicron RBD protein to test against a Wuhan RBD protein in an ELISA assay to determine the efficacy of a COVID-19 treatment that I was working on. AcroBio's Omicron RBD protein coats very well to the plate, and I achieved satisfactory results that were statistically consistent with the results that I have always received with the Wuhan RBD protein. I would recommend ordering from AcroBio- not only was my protein well-packaged in dry ice and delivered in a timely manner, but their customer service is amazing and even personally called me to check up on the status of the package.
We have used many of the ACRO Biosystem's receptor binding domain proteins for assessment of nanobody binding. In biolayer interferometry assessments we have found the quality and purity of the proteins to provide reliable affinity measurements (https://www.biorxiv.org/content/10.1101/2021.11.10.468147v1) of the B.1.617.2 and other variants of concern derived RBDs. ACRO has also provided rapid availability as VoCs have emerged which has proven extremely useful in characterization of nanobody candidates. Continued production of VoC antigens will aid in additional investigations and advancements as the pandemic progresses.
I have recently ordered "SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified) cat: SPD-C52H3". This protein worked fine for me. I am totally satisfied with the process of ordering, the technical support and the lead time taken to deliver the product. The technical data sheet is very well organized and it has all the information needed. Right from molecular weight (calculated and actual under reducing conditions) to directions for reconstitution and storage. My overall experience is excellent with Acrobisystem. With that I am going to order more of the recombinant proteins in the future. Thanks for the service.
Thank you so much for being accommodating and sending the protein to us as fast a possible. We really needed this for an important experiment and I appreciate how well your customer service handled my order. The reply for the invoice went very smoothly as well due to receiving it the same day as the order. I will definitely look for supplies through your company in the future. Overall, I would give Acro Biosystems for my unique protein needs in the future. Thank you.
My peptides arrived in a timely manner and I had no issues using them in my COVID assay. I would purchase again from ACRO due to their great product quality and customer service. The team was very helpful in answering any questions I had about the product and assured me if any issues did come about I could reach out anytime.
Working on COVID-19, Acrobiosystems technical service was very quick and precise to answer my question and the customer service department very reactive.
The product SPD-C5256 and SPD-C525e were very good for in vitro testing, reproducible and acurate on binding assay with different proteins and antibodies which were able to determin Kds for each one of them.