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Monoclonal Anti-RSV-post-F0 specific Antibody, Mouse IgG1 (1A2D9D8) (MALS verified)

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Cat. No. / Size
Price
Qty
RSV-Y180-100ug
$415.00
RSV-Y180-1mg (500ug X 2)
$2735.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Source

    Monoclonal Anti-RSV-post-F0 specific Antibody, Mouse IgG1 (1A2D9D8) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • Clone

    1A2D9D8

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant HRSV (A) Fusion glycoprotein F0 derived from human 293 cells

  • Specificity

    This product is a specific antibody specifically reacts with Postfusion glycoprotein F0/post-F protein (RSV).

  • Application

    Application
    Recommended Usage
    ELISA
    0.5-500 ng/mL
  • Purity

    95% as determined by SDS-PAGE.

    95% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

    For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Postfusion glycoprotein F0/post-F protein (RSV) SDS-PAGE

    Monoclonal Anti-RSV-post-F0 specific Antibody, Mouse IgG1 (1A2D9D8) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Postfusion glycoprotein F0/post-F protein (RSV) SEC-MALS

    The purity of Monoclonal Anti-RSV-post-F0 specific Antibody, Mouse IgG1 (1A2D9D8) (Cat. No. RSV-Y180) is more than 95% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Postfusion glycoprotein F0/post-F protein (RSV) ELISA

    Immobilized HRSV (A) Post-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-post-F0 specific Antibody, Mouse IgG1 (1A2D9D8) (Cat. No. RSV-Y180) with a linear range of 0.488-62.5 ng/mL. The antibody does not bind HRSV (A) Pre-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H7) (QC tested).

    Protocol

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Background

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation.

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