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Product Details
Source
Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
Clone
12C6
Species
Mouse
Isotype
Mouse IgG1 | Mouse Kappa
Conjugate
Unconjugated
Antibody Type
Hybridoma Monoclonal
Reactivity
Virus
Immunogen
Recombinant HRSV (A) Fusion glycoprotein F0 derived from human 293 cells
Specificity
This product is a specific antibody specifically reacts with Prefusion glycoprotein F0/pre-F protein (RSV).
Application
ApplicationRecommended UsageELISA0.2-100 ng/mLPurity
95% as determined by SDS-PAGE.
95% as determined by SEC-HPLC.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70 °C for 3 months after reconstitution;
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Performance Data
SDS-PAGE
Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) (Cat. No. RS0-Y132) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized HRSV (A) Pre-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) (Cat. No. RS0-Y132) with a linear range of 0.098-3.125 ng/mL (QC tested). The antibody does not bind HRSV (A) Post-fusion glycoprotein F0, His Tag (MALS verified) (Cat. No. RSF-V52H6) (Routinely tested).
Protocol
Immobilized HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) (Cat. No. RS0-Y132) with a linear range of 0.5-8 ng/mL. HRSV (B) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H9) is verified not recoginized by Monoclonal Anti-RSV-Pre-F0 specific Antibody, Mouse IgG1 (12C6) (Cat. No. RS0-Y132) (Routinely tested).
Protocol
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