Save Product
Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-18Format96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity＜37.5 pg/mLStandard Curve Range37.5 pg/mL-1200 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of Human IL-18 in cell culture supernates.Sample volume100 uLProduct Overview
resDetect™ Human Interleukin-18 (IL-18) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-18 in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-18 (ACROBiosystems, cat#GMP-L18H16) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of human IL-18.
Application
The kit is developed for the detection and quantitative determination of IL-18 in cell culture supernates, serum, and plasma.
It is for research use only.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES099-C01Pre-coated Anti-IL-18 Antibody Microplate1 plate(8×12 strips)RES099-C02Human IL-18 Standard15 μgRES099-C03Biotin-Anti-IL-18 Antibody15 μgRES099-C04Streptavidin-HRP50 μLRES099-C0510×Washing Buffer50 mLRES099-C062×Dilution Buffer50 mLRES099-C07Substrate Solution12 mLRES099-C08Stop Solution7 mLACRO Quality Management System
Customers Also Viewed
Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human IL-18 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Intra-Assay Statistics
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Recovery
Five parts of blank T cell culture supernatant were added with different concentrations of human IL-18, and the T cell culture supernatant without human IL-18 was used as background to calculate the recovery rate. The range of the recovery rate is 82.6-107.6%, and the average recovery is 94.5%.
Customer Reviews Writing Reviews
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
