My Cart

Human IL-8 ELISA Kit

Customer Reviews
Cat. No. / Size
Price
Qty
CRS-B004-96tests
$515.00
CRS-B004-480tests (96tests X 5)
$2310.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Product Overview

    Human IL-8 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-8 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    IL-8
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    5.0 pg/mL
    Assay Time
    1.75 hr
    Sample volume
    50 μL
    Range
    6.25 pg/mL-800 pg/mL
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.
    NIBSC Code
    89/520

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human IL-8 in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    IL-8 Workflow

  • Storage

    Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CRB004-C01
    Pre-coated Anti-IL-8 Antibody Microplate
    1 plate
    CRB004-C02
    Human IL-8 Standard
    20 μg×2
    CRB004-C03
    Biotin-Anti-IL-8 Antibody Con. Solution
    100 μL
    CRB004-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CRB004-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CRB004-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CRB004-C07
    20×Washing Buffer
    50 mL
    CRB004-C08
    1×Dilution Buffer
    15 mL×2
    CRB004-C09
    Substrate Solution
    12 mL
    CRB004-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • Typical Data 

    Please refer to DS document for the assay protocol.

     IL-8 TYPICAL DATA

    For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B004 is less than 5.0 pg/mL.

Validation
  • Sample Values

    Cell Culture Supernatant: Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using 10 μg/mL PHA, then detected IL-8 secretion amount on Day1, Day 3 and Day 5.

     IL-8 SAMPLE VALUES

    Serum Sample: two hundred and forty healthy serum samples were evaluated for the concentrations of human IL-8 in assay.

     IL-8 SAMPLE VALUES
  • Recovery

    Recombinant IL-8 was spiked into five human serum samples, and then analyzed. The average recovery of IL-8 for serum samples is 94.64%.

     IL-8 RECOVERY

Customer Reviews
Writing Reviews

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-8

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    6 Details

  • Highest Development Stage:

    Approved

Contact Us
Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
Contact Us

[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521

[North America]: 1 Innovation Way, Newark, DE 19711, USA
[Switzerland]: ACRObiosystems AG, c/o Zug Dammstrasse Centre AG, Dammstrasse 19, 6300 Zug Switzerland