Product Details
Product Overview
Human IL-8 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-8 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-8Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity5.0 pg/mLAssay Time1.75 hrSample volume50 μLRange6.25 pg/mL-800 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code89/520Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human IL-8 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow

Storage
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCRB004-C01Pre-coated Anti-IL-8 Antibody Microplate1 plateCRB004-C02Human IL-8 Standard20 μg×2CRB004-C03Biotin-Anti-IL-8 Antibody Con. Solution100 μLCRB004-C04Biotin-Antibody Dilution Buffer8 mLCRB004-C05Streptavidin-HRP Con. Solution500 μLCRB004-C06Streptavidin-HRP Dilution Buffer15 mLCRB004-C0720×Washing Buffer50 mLCRB004-C081×Dilution Buffer15 mL×2CRB004-C09Substrate Solution12 mLCRB004-C10Stop Solution6 mLACRO Quality Management System
Customers Also Viewed
Performance Data
Typical Data
Please refer to DS document for the assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B004 is less than 5.0 pg/mL.
Sample Values
Cell Culture Supernatant: Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using 10 μg/mL PHA, then detected IL-8 secretion amount on Day1, Day 3 and Day 5.

Serum Sample: two hundred and forty healthy serum samples were evaluated for the concentrations of human IL-8 in assay.

Recovery
Recombinant IL-8 was spiked into five human serum samples, and then analyzed. The average recovery of IL-8 for serum samples is 94.64%.

Customer Reviews Writing Reviews

Recent Advances
- English Name:
Interleukin-8
- Category:
- Approved Drugs:
1 Details
- Drugs in Clinical Trials:
6 Details
- Highest Development Stage:
Approved
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521




















