Rabies virus (strain CVS-11) Glycoprotein G, His Tag (MALS verified)

Synonym

Glycoprotein/G Protein (RABV)

Source

Rabies virus (strain CVS-11) Glycoprotein G, His Tag (RAG-V55H5) is expressed from Baculovirus-Insect cells. It contains AA Lys 20 - Lys 458 (Accession # ADJ29911.1).

Predicted N-terminus: Lys 20

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 53.6 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Within the expressed region, several mutations were introduced to optimize the protein expression and stability.

The protein is designed as a trimer.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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